human yap1  (OriGene)

Journal: Journal of Animal Science and Biotechnology

Article Title: Kynurenine promotes porcine intestinal epithelial cell proliferation by activating the AHR-MST1-YAP1 axis

doi: 10.1186/s40104-026-01368-0

Figure Lengend Snippet: L-Trp promotes proliferation of IPEC-J2 cells. A The mRNA expression of proliferative genes PCNA , SYR and MUC13 in IPEC-J2 cells treated with different concentrations of L-Trp ( n = 6). a–c Mean values with unlike letters were significantly different ( P < 0.05). B and C EdU staining and quantification of the ratio of EdU-positive cells in IPEC-J2 cells treated with 4 mmol/L L-Trp or 5 mmol/L L-Glu. D The mRNA expression of cell proliferation associated genes Cyclin E1, Cyclin D1, TGF-β , SMAD4, MST1, YAP1 in IPEC-J2 cells after 4 mmol/L L-Trp or 5 mmol/L L-Glu treatment ( n = 6). Asterisks denote statistically significant differences ( * P < 0.05, ** P < 0.01). Error bars denote SEM

Article Snippet: For inhibiting YAP1 and AHR expression, 0.5 μmol/L Verteporfin ( HY-B0146, MedChemExpress; dissolved in 0.1% DMSO) and 0.2 μmol/L AHR inhibitor CH-223191 (HY-12684, MedChemExpress, USA; dissolved in complete medium) were co-incubated with 4 mmol/L L-kynurenine for 24 h, respectively.

Techniques: Expressing, Staining

Journal: Journal of Animal Science and Biotechnology

Article Title: Kynurenine promotes porcine intestinal epithelial cell proliferation by activating the AHR-MST1-YAP1 axis

doi: 10.1186/s40104-026-01368-0

Figure Lengend Snippet: Kyn promotes the proliferation of IPEC-J2 cells via activating the AHR-MST1-YAP1 axis. A Quantification of the mRNA expression of AHR and CYP1A1 in IPEC-J2 cells after Kyn or Kyn + Verteporfin treatment ( n = 6). B Illustration of the AHR binding sites in the promote region of the MST1 gene. C Quantification of the mRNA expression of MST1 , YAP1 , PCNA and Cyclin E1 after Kyn or Kyn + Verteporfin treatment ( n = 6). D, E EdU staining and scratch assay in IPEC-J2 cells treated with Kyn alone or Kyn + Verteporfin (Scale bar: 20 μm). F Quantification of the ratio of EdU-positive cells and the width of cell migration in IPEC-J2 cells treated with Kyn alone or Kyn + Verteporfin. G and H Quantification of the mRNA expression ( n = 6) of AHR and CYP1A1 and the protein expression ( n = 3) of PCNA in IPEC-J2 cells treated with Kyn alone or Kyn + AHR inhibitor. I Quantification of the mRNA expression of MST1 , YAP1 , PCNA and Cyclin E1 after Kyn or Kyn + AHR inhibitor treatment ( n = 6). J and K Immunostaining and scratch assay in IPEC-J2 cells treated with Kyn alone or Kyn + AHR inhibitor (Scale bar: 20 μm). The yellow arrowheads indicated the nuclear localization of AHR, while the red arrowheads indicated the cytoplasmic localization of AHR, respectively. L Quantification of the fluorescence intensity of PCNA and the width of cell migration in IPEC-J2 cells after Kyn or Kyn + AHR inhibitor treatment. Asterisks denote statistically significant differences ( * P < 0.05, ** P < 0.01). Error bars denote SEM

Article Snippet: For inhibiting YAP1 and AHR expression, 0.5 μmol/L Verteporfin ( HY-B0146, MedChemExpress; dissolved in 0.1% DMSO) and 0.2 μmol/L AHR inhibitor CH-223191 (HY-12684, MedChemExpress, USA; dissolved in complete medium) were co-incubated with 4 mmol/L L-kynurenine for 24 h, respectively.

Techniques: Expressing, Binding Assay, Staining, Wound Healing Assay, Migration, Immunostaining, Fluorescence

Journal: Journal of Animal Science and Biotechnology

Article Title: Kynurenine promotes porcine intestinal epithelial cell proliferation by activating the AHR-MST1-YAP1 axis

doi: 10.1186/s40104-026-01368-0

Figure Lengend Snippet: Kyn-induced intestinal organoids proliferation is dependent on the activation of the MST1-YAP1 pathway. A – D Representative images of porcine intestinal organoids and the quantification of budding efficiency from the control, Kyn and Kyn + Verteporfin treated groups, respectively (scale bar: 100 μm). E–H Immunostaining and the fluorescence intensity of OLFM4 in the control, Kyn and Kyn + Verteporfin treated groups, respectively (scale bar: 20 μm). I and J The protein expression of PCNA, AHR, MST1 and Cyclin D1 in intestinal organoids treated with Kyn or Kyn + Verteporfin ( n = 3). K Proposed model of Trp metabolism-derived Kyn in regulating intestinal epithelial cell proliferation via activation of the AHR-MST1-YAP axis. Trp, tryptophan; Kyn, kynurenine; AHR, aryl hydrocarbon receptor; ARNT, aryl hydrocarbon receptor nuclear translocator; ISC, intestinal stem cell; IEC, intestinal epithelial cell. Asterisks denote statistically significant differences ( * P < 0.05, ** P < 0.01). Error bars denote SEM

Article Snippet: For inhibiting YAP1 and AHR expression, 0.5 μmol/L Verteporfin ( HY-B0146, MedChemExpress; dissolved in 0.1% DMSO) and 0.2 μmol/L AHR inhibitor CH-223191 (HY-12684, MedChemExpress, USA; dissolved in complete medium) were co-incubated with 4 mmol/L L-kynurenine for 24 h, respectively.

Techniques: Activation Assay, Control, Immunostaining, Fluorescence, Expressing, Derivative Assay

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: ( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Western Blot, Expressing, Staining

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: YAP1 protein levels are significantly downregulated in the nuclear fractions but not the cytosolic fractions of the AD brains relative to NC brains. Brain lysates were immunoblotted, and protein levels were quantified by normalizing to HDAC2 levels for nuclear fractions and to GAPDH levels for cytosolic fractions. Data are mean ± SEM ( n = 5 per group). *** p < 0.001 by t -test.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques:

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: Antibody-based multiplex microarray screening of 8000 proteins revealed alterations of specific proteins. ( A ) SH-SY5Y cells expressing YAP1-GFP. Scale, 50 µm. ( B ) YAP1-GFP expression supported by immunoblot. ( C ) Examples of glass slide luminescence. ( D ) Heatmap of control and YAP1 biomarkers in 8000 samples. The data were imported to JMP Genomics 10.2 for Hierarchical Cluster analysis.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Multiplex Assay, Microarray, Expressing, Western Blot, Control

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: YAP1 expression increased markers of synaptic endocytosis and ATP-dependent activity. ( A ) Gene ontology (GO) biological process over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) GO cellular component over-representation analysis with 283 differentially expressed biomarkers between control and YAP1-expressing SH-SY5Y cells.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Expressing, Activity Assay, Control

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: ( A ) GO molecular function over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) Interaction network of human YAP1 protein as analyzed by STRING.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Control

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: The bar graph shows GO analysis results of the increased ( A ) and decreased proteins ( B ) due to YAP1 expression using ShinyGO 0.85.1. Sets of nonredundant, significant GO enrichment terms are displayed to show the foldenrichment and the number of proteins matched to each term. The fold-enrichment values are displayed with the highest on top, and the shade of each bar reflects the number of proteins found in a given GO category.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Expressing

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: Evidence of increased levels of ( A ) NEDD9 and ( B ) ARHGEF1 inYAP1-GFP-expressing SH-SY5Y cells when compared to control cells by immunoblotting. ( C ) The LC3-16/LC3-18 ratio was also increased, indicating YAP1 activates autophagy. However, levels of ( D ) p16INK4a, a marker of senescence, were almost completely abolished. Data are mean + SEM, n = 3/group. *, p < 0.05, **, p < 0.01, ***, p < 0.001 by t -test.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Expressing, Control, Western Blot, Marker

Journal: bioRxiv

Article Title: The regulatory landscape of optic fissure closure in the vertebrate eye

doi: 10.64898/2026.02.11.705341

Figure Lengend Snippet: ( A-D ) Number of peaks and highest match de novo motifs identified by HOMER for each context specific category. Only motifs with a percentage of target to background ratio > 1.5 were included. ( E-H ) Histograms showing motif occurrence distribution around the centre of peaks corresponding to analysis in A-D. Lines without observable enrichment are translucent. Motif consensus sequences and their likely TF identities are shown below and heights correlate to the frequency of base occurrence. ( I ) YAP1 immunofluorescence during chicken OFC at HH30 showed highest signal (arrows) in the edges of the fusing OFM and in the fused margins. In contrast, low signal was detected in dorsal tissue. Abbreviations : RPE, retinal pigmented epithelium, NR, neural retina; POM, periocular mesenchyme.

Article Snippet: Immunofluorescence of YAP1 was performed using methods described previously , but using rabbit anti-human YAP1 antibody (D8H1X, Cell Signalling Technology) diluted 1:50 in block solution (PBS, 0.1% BSA, 0.05% Triton X-100) and incubated overnight at 4°C, and detected with Alexa-fluor 488nm goat anti-rabbit secondary antibody (1:1000 dilution; A11037, Thermo Fisher) and DAPI (1:1000 dilution; D3571, Thermo Fisher).

Techniques: Immunofluorescence

Journal: bioRxiv

Article Title: The regulatory landscape of optic fissure closure in the vertebrate eye

doi: 10.64898/2026.02.11.705341

Figure Lengend Snippet: (A) Shared sequence similarities for homeodomain motifs LHX2, VAX1, ALX1 and LHX2. ( B ) TPM plot showing expression patterns of TEAD1 , TEAD3 , TEAD4 and YAP1 from the RNA-seq analysis . (C) TPM of transcription factors expression levels from the RNA-seq data. Only values for transcription factor genes with a motif enrichment indicated by HOMER were plotted.

Article Snippet: Immunofluorescence of YAP1 was performed using methods described previously , but using rabbit anti-human YAP1 antibody (D8H1X, Cell Signalling Technology) diluted 1:50 in block solution (PBS, 0.1% BSA, 0.05% Triton X-100) and incubated overnight at 4°C, and detected with Alexa-fluor 488nm goat anti-rabbit secondary antibody (1:1000 dilution; A11037, Thermo Fisher) and DAPI (1:1000 dilution; D3571, Thermo Fisher).

Techniques: Sequencing, Expressing, RNA Sequencing

Journal: Communications Biology

Article Title: Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling

doi: 10.1038/s42003-025-09210-5

Figure Lengend Snippet: A qPCR was performed to assess FOXO1 expression in TDSCs after treatment with exosomes derived from miR-212-5p -depleted ADSCs. B Western blot was conducted to evaluate FOXO1 and p-YAP1 levels after treatment with exosomes derived from miR-212-5p-depleted ADSCs. C Bioinformatics analysis using the Starbase database was performed to predict the target interaction between miR-212-5p and FOXO1 . D qPCR was used to measure miR-212-5p levels in TDSCs after transfection with miR-212-5p mimics. E Dual-luciferase reporter assay was used to assess luciferase activity after treatment with exosomes derived from miR-212-5p -depleted or miR-212-5p -overexpressing ADSCs. F RNA pull-down assay was conducted using biotin-labeled miR-212-5p to evaluate its binding interaction with FOXO 1. G qPCR was performed to assess FOXO 1 expression after transfection with shFOXO1 in ADSCs. H Western blot analysis was used to evaluate FOXO1 and p-YAP1 levels after knockdown of miR-212-5p in ADSC-Exos and simultaneous knockdown of FOXO1. Data are presented as mean ± SD, and statistical analysis was conducted using a student t-test (for D , F – G ) of one-way ANOVA followed by Tukey’s post hoc test (for A , B , E , H ). n = 3.*p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The cDNA ORF coding full sequences for YAP1 (Origene, Shanghai, China) mutant serine-to-alanine mutation at position 127 (S127A) was generated through PCR-based site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, Sacramento, CA, USA) following the manufacturer ́s protocol, and was subcloned into an overexpressing vector.

Techniques: Expressing, Derivative Assay, Western Blot, Transfection, Luciferase, Reporter Assay, Activity Assay, Pull Down Assay, Labeling, Binding Assay, Knockdown

Journal: Communications Biology

Article Title: Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling

doi: 10.1038/s42003-025-09210-5

Figure Lengend Snippet: A , B qPCR and Western blot were performed to analyze PP1A levels in TDSCs transfected with shNC or shFOXO1 after treatment with exosomes derived from miR-212-5p-depleted ADSCs. C The JASPAR database was used to predict FOXO1 binding sites on the PP1A promoter. D ChIP assay was conducted using a FOXO1 antibody to assess its binding to the PP1A promoter. E , F qPCR and Western blot were performed to evaluate FOXO1 expression in TDSCs after transfection with FOXO1 overexpression vectors. G Dual-luciferase reporter assay was used to investigate the impact of FOXO1 knockdown and overexpression on PP1A transcriptional activity. H qPCR was performed to assess PP1A expression following FOXO1 knockdown and overexpression in TDSCs. I qPCR was conducted to evaluate PP1A expression after transfection with shPP1A in TDSCs. J Western blot was performed to study the effects of FOXO1 and PP1A knockdown on PP1A and p-YAP1 levels. K Immunofluorescence was used to examine nuclear YAP1 levels (green) in TDSCs after FOXO1 and PP1A knockdown. Scale bar: 100 μm. Data are presented as mean ± SD, and statistical analysis was conducted using a student t-test (for D – F , I ) of one-way ANOVA followed by Tukey’s post hoc test (for A , B , G , H , J ). n = 3 **p < 0.01, ***p < 0.001.

Article Snippet: The cDNA ORF coding full sequences for YAP1 (Origene, Shanghai, China) mutant serine-to-alanine mutation at position 127 (S127A) was generated through PCR-based site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, Sacramento, CA, USA) following the manufacturer ́s protocol, and was subcloned into an overexpressing vector.

Techniques: Western Blot, Transfection, Derivative Assay, Binding Assay, Expressing, Over Expression, Luciferase, Reporter Assay, Knockdown, Activity Assay, Immunofluorescence

Journal: Communications Biology

Article Title: Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling

doi: 10.1038/s42003-025-09210-5

Figure Lengend Snippet: A Western blot was performed to analyze the effects of transfection with wild-type YAP1 (YAP1-WT) expression vectors or S127A mutant YAP1 expression vectors (YAP1-S127A) on YAP1 and p-YAP1 levels. B – F MTT, EdU staining, scratch assay, Transwell assay, and Western blot were used to evaluate TDSC proliferation, migration, and the expression of tenogenic differentiation markers, including TNC, TNMD, Scx, COL1A1, and GAPDH, after FOXO1 overexpression with simultaneous overexpression of the S127A mutant YAP1. Scale bar: 100 or 500 μm. G Alcian Blue staining was performed to evaluate cartilage-like matrix deposition in TDSCs. Scale bar: 100 μm. Data are presented as mean ± SD, and statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test. n = 3. **p < 0.01, ***p < 0.001.

Article Snippet: The cDNA ORF coding full sequences for YAP1 (Origene, Shanghai, China) mutant serine-to-alanine mutation at position 127 (S127A) was generated through PCR-based site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, Sacramento, CA, USA) following the manufacturer ́s protocol, and was subcloned into an overexpressing vector.

Techniques: Western Blot, Transfection, Expressing, Mutagenesis, Staining, Wound Healing Assay, Transwell Assay, Migration, Over Expression

Journal: Communications Biology

Article Title: Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling

doi: 10.1038/s42003-025-09210-5

Figure Lengend Snippet: A Hematoxylin and eosin (HE) staining was performed to evaluate the arrangement of fibrous tissue in tendons from mice treated with exosomes derived from normal or miR-212-5p -depleted ADSCs. Scale bar: 50 or 20 μm. B Safranin O/Fast Green staining of tendon sections to evaluate ectopic cartilage-like matrix formation after injury in different groups of mice. Scale bar: 50 or 20 μm. C Immunohistochemistry (IHC) was used to assess the levels of TNMD, Scx, COL1A1, and SOX9 in tendon tissues from different groups of mice. Scale bar: 20 μm D , E Western blot analysis was conducted to examine the expression of tenogenic differentiation markers, including TNC, TNMD, Scx, and COL1A1, as well as PP1A, FOXO1, and p-YAP1 levels in tendon tissues from different groups of mice. F , G Biomechanical testing was performed to measure the failure load and stiffness of tendon tissues from different groups of mice. Data are presented as mean ± SD, and statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test. n = 6. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The cDNA ORF coding full sequences for YAP1 (Origene, Shanghai, China) mutant serine-to-alanine mutation at position 127 (S127A) was generated through PCR-based site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, Sacramento, CA, USA) following the manufacturer ́s protocol, and was subcloned into an overexpressing vector.

Techniques: Staining, Derivative Assay, Immunohistochemistry, Western Blot, Expressing