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yap1 human sirna oligo duplex  (OriGene)


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    OriGene yap1 human sirna oligo duplex
    Yap1 Human Sirna Oligo Duplex, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yap1 human sirna oligo duplex/product/OriGene
    Average 90 stars, based on 1 article reviews
    yap1 human sirna oligo duplex - by Bioz Stars, 2026-03
    90/100 stars

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    Effect of AMF30a on cell adhesion of corneal endothelial cells. ( A - C ) Cell adhesion assay was performed using crystal violet assay. Scale bar = 500 μm. ( D ) immunofluorescence staining of E-cadherin are shown. Scale bar = 100 μm. ( E and F ) F-actin was visualized using phalloidin staining. Scale bar = 100 μm. ( G and H ) pERK1/2 levels were evaluated using Western blot. ( I and J ) pYAP levels were evaluated using western blot. ( K and L ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 50 μm. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: AMF30a promotes survival and function of human corneal endothelial cells by regulating TGF-β/ROCK/HIPPO pathway

    doi: 10.1038/s41598-025-13656-2

    Figure Lengend Snippet: Effect of AMF30a on cell adhesion of corneal endothelial cells. ( A - C ) Cell adhesion assay was performed using crystal violet assay. Scale bar = 500 μm. ( D ) immunofluorescence staining of E-cadherin are shown. Scale bar = 100 μm. ( E and F ) F-actin was visualized using phalloidin staining. Scale bar = 100 μm. ( G and H ) pERK1/2 levels were evaluated using Western blot. ( I and J ) pYAP levels were evaluated using western blot. ( K and L ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 50 μm. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

    Article Snippet: Primary antibodies were as follows: rabbit anti-human CD166 antibody (ab109215, Abcam, 1∶500 dilution), rabbit anti-human ZO-1 antibody (sc-10804, Santa Cruz, 1∶500 dilution), mouse anti-human ERK1/2 antibody (sc-514032, Santa Cruz, 1∶500 dilution); mouse anti-human pERK1/2 antibody (sc-136521, Santa Cruz, 1∶500 dilution); mouse anti-human YAP antibody (sc-376830, 1∶500 dilution); rabbit anti-pYAP antibody (PA5-17481, Invitrogen, 1∶500 dilution); rabbit anti-human PAD2 antibody (12110-1-AP, Proteintech, 1∶500 dilution); mouse anti-ATF4 antibody (sc-390063, 1∶500 dilution); mouse anti-human ROCK1 antibody (sc-17794, 1∶500 dilution); mouse anti-ROCK2 antibody (sc-398519, 1∶500 dilution); or rabbit anti-GAPDH antibody (LF-PA0212, Abfrontier, 1∶5000 dilution).

    Techniques: Cell Adhesion Assay, Crystal Violet Assay, Immunofluorescence, Staining, Western Blot, Translocation Assay

    Effect of AMF30a on TGF-β-mediated ROCK/HIPPO signaling pathway of corneal endothelial cells. ( A and B ) pERK1/2 levels were evaluated using western blot. ( C and D ) pYAP levels were evaluated using western blot assays. ( E and F ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 100 μm. ( G - I ) ROCK1 and ROCK2 levels were evaluated by western blotting. (J and K) Nuclear translocation of NF-κB was evaluated using immunofluorescence staining. Scale bar = 100 μm. (L and M) ATF4 levels were evaluated by western blotting. * p < 0.05, and ** p < 0.01.

    Journal: Scientific Reports

    Article Title: AMF30a promotes survival and function of human corneal endothelial cells by regulating TGF-β/ROCK/HIPPO pathway

    doi: 10.1038/s41598-025-13656-2

    Figure Lengend Snippet: Effect of AMF30a on TGF-β-mediated ROCK/HIPPO signaling pathway of corneal endothelial cells. ( A and B ) pERK1/2 levels were evaluated using western blot. ( C and D ) pYAP levels were evaluated using western blot assays. ( E and F ) Nuclear translocation of YAP was evaluated using immunofluorescence staining. Scale bar = 100 μm. ( G - I ) ROCK1 and ROCK2 levels were evaluated by western blotting. (J and K) Nuclear translocation of NF-κB was evaluated using immunofluorescence staining. Scale bar = 100 μm. (L and M) ATF4 levels were evaluated by western blotting. * p < 0.05, and ** p < 0.01.

    Article Snippet: Primary antibodies were as follows: rabbit anti-human CD166 antibody (ab109215, Abcam, 1∶500 dilution), rabbit anti-human ZO-1 antibody (sc-10804, Santa Cruz, 1∶500 dilution), mouse anti-human ERK1/2 antibody (sc-514032, Santa Cruz, 1∶500 dilution); mouse anti-human pERK1/2 antibody (sc-136521, Santa Cruz, 1∶500 dilution); mouse anti-human YAP antibody (sc-376830, 1∶500 dilution); rabbit anti-pYAP antibody (PA5-17481, Invitrogen, 1∶500 dilution); rabbit anti-human PAD2 antibody (12110-1-AP, Proteintech, 1∶500 dilution); mouse anti-ATF4 antibody (sc-390063, 1∶500 dilution); mouse anti-human ROCK1 antibody (sc-17794, 1∶500 dilution); mouse anti-ROCK2 antibody (sc-398519, 1∶500 dilution); or rabbit anti-GAPDH antibody (LF-PA0212, Abfrontier, 1∶5000 dilution).

    Techniques: Western Blot, Translocation Assay, Immunofluorescence, Staining